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Product title :

Footrot on a sheep breeding farm in the Himalayan state of Jammu and Kashmir

Author(s) : A. Taku, R. Chhabra & B.A. War

Summary :

In the present study ovine footrot was detected clinically on a sheep farm in the Himalayan state of Jammu and Kashmir. Dichelobacter nodosus was confirmed by culture and polymerase chain reaction (PCR) using species-specific 16S ribosomal RNA primers. When cultured, the organism appeared as flat colourless colonies having a fine granulated structure with irregular margins, and showing characteristic Gram-negative rods with swollen ends. Detection by PCR from cultured bacteria resulted in amplification of a 783 base pairs (bp) product. Serogrouping by multiplex PCR using group (A-I)-specific primers revealed the presence of serogroup B-specific bands of 283 bp.
 
Keywords
16S rRNA – Dichelobacter nodosus fimA gene – Footrot – Jammu and Kashmir – Sheep.

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