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Product title :

Influence of the incubation temperature and the batch components on the sensitivity of an enzyme-linked immunosorbent assay to detect Aujeszky’s disease virus glycoprotein E (gE)

Author(s) : A.B. Cay & Y. Van der Stede

Summary :

Although licensed batches of an enzyme-linked immunosorbent assay (ELISA) for Aujeszky’s disease virus (ADV) were used, and the assays were performed within an ISO/IEC 17025 accredited quality control system, certain routine runs of the ADV ELISA were not validated using the quality system criteria, even when all technical parameters were controlled. Incubation at different temperatures and batch composition were identified as parameters that could result in non-validated assays/runs. Therefore, the effect of incubation temperature and batch composition on the analytical sensitivity of the ELISA was investigated. The World Organisation for Animal Health (OIE) standard reference serum ADV1 was diluted 1:8 and tested in 94 different glycoprotein E ELISA runs performed with different batches and different incubation temperatures. The incubation temperature and batch components had a significant influence on the qualitative result for the OIE standard reference serum. An incubation temperature of at least 22°C was recommended, based on the results of this analysis.
 
Keywords
Anti-ADV gE enzyme-linked immunosorbent assay – Aujeszky’s disease virus – Batch control – Sensitivity.

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