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Bovine herpesvirus 1: within-herd seroprevalence and antibody levels in bulk-tank milk
|Author(s) :||S. Martínez, E. Yus, M.L. Sanjuán, F. Camino, M.C. Eiras, I. Arnaiz & F.J. Diéguez|
The aim of the present study was to establish a relationship between the results obtained with the enzyme-linked immunosorbent assay (ELISA) technique for antibodies (against bovine herpesvirus 1) in serum and those in milk at the herd level. For this purpose, 275 samples of bulk-tank milk were analysed with glycoprotein E (gE) antibody ELISA and 207 more were analysed with glycoprotein B (gB) antibody ELISA (482 in total). All of these samples came from dairy herds whose seroprevalence was also evaluated. The results of this study were then used to analyse the sensitivity of the bulk-tank milk test in detecting herds with a high risk of active infection (>60% seroprevalence) and its specificity in detecting those with few (<20%) or no seropositive animals. In regard to the reference test (results in blood serum), the sensitivity of the bulk-tank milk test in detecting herds with >60% seropositive animals was 100% for both gE and gB ELISAs.
Bovine herpesvirus 1 – Bulk-tank milk – Enzyme-linked immunosorbent assay – Glycoprotein B – Glycoprotein E – Serology – Within-herd seroprevalence.