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Product title :

The equine arteritis virus isolate from the 2010 Argentinian outbreak

Author(s) : G.E. Metz, M.S. Serena, C.J. Panei, E.O. Nosetto & M.G. Echeverría

Summary :

No. 22102014-00048-EN

A semen sample from a stallion infected during the 2010 equine arteritis virus (EAV) outbreak was received for viral isolation prior to castration of the animal. The virus was identified using a polyclonal antibody immunofluorescence test. Reverse-transcription polymerase chain reaction (RT-PCR) was used to amplify a region of the GP5 gene with primers GL105F and GL673R. The PCR products were purified and sequences of both strands were determined in a MegaBACE™ 1000 with inner primers CR2 and EAV32. A phylogenetic dataset was built with the previously reported sequences of five strains isolated in Argentina, together with a group of selected sequences obtained from GenBank.

Keywords
Argentina – Equine arteritis virus – Outbreak – Semen sample.

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