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Product title :

Seroepidemiological survey on Rift Valley fever among small ruminants and their close human contacts in Makkah, Saudi Arabia, in 2011

Author(s) : A.M. Mohamed, A.M. Ashshi, A.H. Asghar, I.H.A. Abd El-Rahim, A.G. El-Shemi & T. Zafar

Summary :

No. 22052014-00032-EN

This study describes a seroepidemiological survey on Rift Valley fever (RVF) among small ruminants and their close human contacts in Makkah, Saudi Arabia. A total of 500 small ruminants (126 local, 374 imported) were randomly selected from the sacrifice livestock yards of Al-Kaakiah slaughterhouse, in the holy city of Makkah, during the pilgrimage season 1432 H (4–9 November 2011). In addition, blood samples were collected from 100 local workers in close contact with the animals at the slaughterhouse. An RVF competition multi-species enzyme-linked immunosorbent assay (ELISA) detecting anti-RVF virus immunoglobulin G (IgG)/immunoglobulin M (IgM) antibodies and an RVF IgM-specific ELISA were used for serological investigations. In total, 84 (16.8%) of the 500 sacrificial sheep and goats tested seropositive in the competition ELISA but no IgM antibodies were detected in the IgM-specific assay. All seropositive samples, comprising 17.91% of the imported animals and 13.49% of the local ones, were therefore designated positive for anti-RVF virus IgG antibody.

Keywords

Epidemiology – Makkah – Pilgrimage – Rift Valley fever – Saudi Arabia – Serological survey – Small ruminant.

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