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Product title :

Isolation of bluetongue virus serotype 1 from aborted goat fetuses

Author(s) : H.C. Chauhan, et al.

Summary :

No. 09052014-00031-EN

Abortions and stillbirths were noticed in pregnant goats on a farm in the state of Gujarat, India. About 50% of the pregnant goats aborted or gave birth to dead kids. Bluetongue virus (BTV) antibody in the sera of affected goats was detected using a competitive enzyme-linked immunosorbent assay (ELISA). Viral antigen in the blood of these goats and in the aborted fetal spleens was detected using a sandwich ELISA. Two viruses (SKN-9, SKN-10) were isolated in cell culture from aborted fetal spleens and were confirmed as Orbivirus by demonstration of ten bands in RNA polyacrylamide gel electrophoresis and identified as BTV-1 by sequencing of the VP2 gene.

Keywords

Abortion – Bluetongue virus serotype 1 – Fetus – Goat – Transplacental transmission – VP2 gene – Wild type.

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