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Molecular characterisation of Brucella species
|Author(s) :||H.C. Scholz & G. Vergnaud|
The genus Brucella (Mayer and Shaw, 1920) currently consists of ten species with validly published names. Within most species further differentiation into biovars exists. Genetically, all Brucella species are highly related to each other, exhibiting sequence similarity values of 98% to 100% in aligned regions (core genome). The population structure is clonal. Despite this close genetic relatedness, the various species can be clearly distinguished from each other by application of high resolution molecular typing tools, in addition to assessment of phenotype and host preference. Accurate species delineation can be achieved by conventional multiplex polymerase chain reaction (PCR), single nucleotide polymorphism (SNP) analysis and multilocus sequence typing (MLST) or multilocus sequence analysis (MLSA). The last is also suitable for phylogenetic reconstructions, owing to the highly clonal evolution of the different species.
Brucella spp. – Clonal population – High-resolution typing assay – Low genetic diversity – Monophyletic genus.