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Product title :

Prevalence of Clostridium perfringens type A isolates in commercial broiler chickens and parent broiler breeder hens in Egypt

Author(s) : K.M. Osman, Y.A. Soliman, Z.M.S. Amin & M.A.K. Aly

Summary :

The aim of this study was to determine the presence of genes coding for alpha (cpα), beta (cpβ), epsilon (εtx), iota (ιA), enterotoxin (cpε) and beta2 (cpβ2) toxins in Clostridium perfringens isolates from broiler chickens and parent broiler breeder hens, using multiplex polymerase chain reaction (PCR) assay. The prevalence of C. perfringens in the intestinal segments and the effects of age were also investigated. The highest isolation rate was from the duodenum, at 41.7% in broiler chickens and 58.4% in parent broiler breeder hens; the lowest isolation rates came from the ileum, at 15.6% and 27.1%, respectively. Chickens harboured C. perfringens in the intestine and this increased with age.
 
Keywords
Breeders – Broilers – Chickens – cpα toxin – cpβ toxin – cpβ2 gene – cpε toxin – Egypt – εtx toxin – ιA toxin – Necrotic enteritis – Poultry.

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